TOPIC

Isolation, Identification and Characterization of Bacteria Capable of Degrading Chlorpyrifos from Agricultural Soil at Amansea, Anambra State Nigeria.

ABSTRACT
Chlorpyrifos (O, O-diethyl O-(3,5,6-trichloro-2-pyridyl phosphorothioate) a broad spectrum moderately toxic organophosphorous insecticide is commonly used as pest control on grain, cotton, fruits, and vegetable crops against pest. Although it is important, its use  has become one of the major causes of soil and water pollution. The present technique for removing chloryrifos insecticide from the environment which is biodegradation has shown to be more effective than any other physical or chemical methods like chemical hydrolysis. Therefore the aim of this research was to evaluate the biodegradation of chlorpyrifos insecticide by bacteria isolated from agricultural soil in Amansea, Anambra State, Nigeria. In the present study, two bacteria capable of degrading chlorpyrifos were isolated from Agricultural soil using mineral salts medium and characterized based on their physiological, biochemical, morphological and 16S rRNA sequencing as strains of Bacillus cereus ST06 and Chryseobacterium sp. 6024. Their growth response in mineral salts medium supplemented with 20mg/l concentration of chlorpyrifos was determined by monitoring the optical density at 600nm and at optimium condition of pH 6.5 and 300C for 28 days. The residual chlorpyrifos concentration after 28 days was also determined using Gas Chromatography- Electron Cathode Detector (GC-ECD). The result from one way ANOVA indicated that there were significant differences (P < 0.05) in the Growth responses to the insecticides by the test organisms. The result showed that Bacillus cereus ST06 and Chryseobacterium sp. 6024 reached maximum growth on 20mg/l chlorpyrifos with an arithmetic mean difference of 0.23±0.20 and 0.42±0.02 respectively on day 16 .There were also significant (<0.05) difference in concentration of residual chlorpyrifos degraded by the isolates obtained from the GC-ECD curves. Bacillus cereus ST06 and Chryseobacterium sp. 6024 degraded 63% and 57% of 20mg/l chlorpyrifos respectively. Bacillus cereus ST06 showed better results and possess potential to be used in biodegradation of 20mg/l Chlorpyrifos than Chryseobacterium sp. 6024. It is therefore recommended that further studies on the effect of varying Chlorpyrifos concentration on the bacteria singly and as a consortium be studied to better understand their potential for degrading chlorpyrifos efficiently.Keywords: Isolation, Identification, Characterization, Bacteria, Biodegradation, Chlorpyrifos and Insecticides

Download: FULL TEXT PDF

PUBLISHED

2023-05-15

HOW TO CITE

Emeribe, Chiemeka Elochi, Onuorah, Samuel Chinedu and Chukwukelo, Chinonyelum Dilys (2023). Isolation, Identification and Characterization of Bacteria Capable of Degrading Chlorpyrifos from Agricultural Soil at Amansea, Anambra State Nigeria. IDOSR JOURNAL OF BIOLOGY,CHEMISTRY AND PHARMACY8(2)102-112. https://doi.org/10.59298/IDOSR/JBCP/23/10.128 

Rate: