The hepatoprotective effect of ethanolic extract of Oyster mushroom (Pleurotus ostreatus) on acutely administered lead poison in albino rats was studied by assaying the concentration of liver markers enzymes: Alanine Transaminase (ALT), Aspartate Transaminase (AST), Alkaline Phosphate (ALP) and Total Bilirubin (TBL), Total Protein (TP), Albumin (ALB). A total of twenty-four albino rats were distributed into six groups of four animals each. Group 1 (control), group 2 (20mg/kg lead alone), group 3 (40mg/kg lead alone), group 4 (250mg/kg mushroom extract alone), group 5 (250mg/kg mushroom and 20mg/kg lead), and group 6 (250mg/kg mushroom and 40mg/kg lead). These animal groups were administered their respective treatments for a period of twenty-one days. Thereafter, various tests were carried out on the liver enzymes. The groups administered with lead (low and high dose) showed significant increase in levels of AST, ALT, ALP & TBL but reduced levels of ALB and TP respectively (59.00±1.63, 23.00±1.47, 61.25±2.49, 16.25±0.62, 31.75±1.18, 46.75±0.85, p <0.05), (63.75±0.48, 29.75±0.85, 72.25±1.03, 18.00±0.41, 27.00±0.71, 36.00±1.47, p<0.05) compared to the control with mean values of 37.75±1.88, 15.50±0.64, 46.50±1.55, 11.75±0.48, 39.00±0.71, 71.50±1.32. On the other hand, groups 4, 5 and 6 treated with oyster mushroom extract showed a hepatoprotective effect by maintaining the level of ALT (14.25±1.11, 16.00±0.91, 20.25±0.48, p<0.05), AST (24.25±1.49, 27.25±1.18, 31.25±1.65, p<0.05), ALP (34.25±1.11, 37.50±2.72, 39.50±0.87, p<0.05), TP (78.25±1.37, 72.25±0.47, 74.25±2.56, p<0.05), ALB (38.75±0.48, 40.00±0.41, 43.25±0.63, P<0.05) and TBL (11.00±0.41, 11.75±0.48, 13.25±0.48 p<0.05), around the normal level. Histological examination of the liver tissues showed that mushroom extract was hepatoprotective against the lead induced alterations, notably inflammation, necrosis and vacuolation. This result reveals that aqueous extract from Pleurotus ostreatus has significant hepatoprotection against lead induced hepatocellular injury.
Keywords: Lead, Pleurotus ostreatus, Hepatoprotection, Hepatotoxicity, AST, ALT, ALP, TP, ALB, TBL
HOW TO CITE
Zitte, Leelee Famii and Tochi Ben (2023). Hepatoprotective Effect of Ethanolic Extract of Oyster Mushroom (Pleurotus ostreatus) on acutely Administered Lead Poison in Albino Rats. IDOSR JOURNAL OF SCIENTIFIC RESEARCH 8(1)26-32,2023.